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Binding of transcription factors measured on individual DNA molecules in living cells. An important question in the field of gene regulation is how a limited set of transcription factors interpret the same genome in different ways to create over cell types found in our bodies, all of which are defined by the expression of a unique set of genes. To achieve this, transcription factors have evolved cooperative mechanisms to enable precise control of gene expression. In a new study, members of the Krebs group in the Genome Biology Unit at EMBL Heidelberg have developed a method to directly visualise how transcription factors co-occupy DNA in living cells, and have used it to better understand their cooperativity mechanisms. Transcription factors are proteins that bind to specific regulatory DNA sequences to control which genes are turned on or off. For transcription factors to activate regulatory regions of the DNA, histones typically need to be removed so the transcription factors can access their recognition sites.

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